In 2024, scientists extracted ancient DNA from 64 skeletons found in a sealed underground chamber (Chultun) near Chichén Itzá's Great Pyramid, revealing that all victims were male children aged 3-6 years old, closely related to each other, contradicting the 400-year-old Spanish colonial account that claimed Maya sacrifice victims were young women. This discovery demonstrates how ancient DNA analysis can correct historical misconceptions and reveals that the Maya performed ritual sacrifices of male twins to symbolically reenact the Hero Twins myth from their creation narrative, representing a deliberate cosmological practice rather than the opportunistic killing of women described in colonial accounts.
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Scientists Were WRONG About The Maya — DNA From Walled Skeletons Proves It本站添加:
Beneath the shadow of Chichenita's Great Pyramid, sealed inside an underground system connected to the Maya underworld, lay the bones of more than a hundred victims. Historians believed they knew who these people were. Scientists believed the same. When an international team extracted ancient DNA from 64 of those skeletons in 2024, the story that had stood for 400 years came apart.
The assumption had roots in conquest. In the 16th century, Spanish colonial authorities dredged the sacred cenote at Chichen it, a natural limestone sinkhole 14 m deep, connected to the city's ceremonial center by a raised stone causeway 300 m long. What they pulled from the water shaped everything that followed. Diego Danda, the Franciscan frier who destroyed most of the Maya written record and then wrote his own account of what he had observed, described young women being thrown into the cenote alive as offerings to the rain god Chak. Subsequent excavations in the early 20th century, including a dredging operation financed by Harvard University's Peabody Museum between 1904 and 1910, recovered gold, jade, ceramics, and human remains. The colonial framing held for four centuries. The Maya sacrifice of young women and girls became one of the most repeated facts in Mesoamerican archaeology. Taught in universities, printed in textbooks, and repeated in every documentary that ever pointed a camera at the pyramid. The story had the weight of conquest behind it. It had never been tested against the bones.
Less than a kilometer from the sacred cenote, during construction of a small airport behind the site in the spring of 1967, excavation workers broke through the floor of an underground sistern. The Chulon, a human-made chamber originally built for storing drinking water, had been converted into something else entirely. Connected to a low ceiling cave, its two chambers held the scattered bones of more than 100 individuals, almost all of them children, covered by undisturbed layers of bark and limestone powder.
Archaeologists had 2 months to excavate before the runway had to be finished.
They removed what they could, cataloged the bones, and stored them nearby. The question of who these children were would wait nearly six decades for an answer.
Rodrigo Barera, an archogeneticist at the Maxplank Institute for Evolutionary Anthropology in Leipzk, and his colleagues began extracting ancient DNA from the prois bone of each skeleton, the dense pyramid-shaped structure at the base of the skull that houses the inner ear and the portion of the human skeleton that preserves genetic material better than any other in warm, humid burial conditions. Katherine Nagel, study co-author and archiogeneticist at the same institute, directed the sampling protocol using only the left petrus bone from each individual to ensure no skeleton was counted twice.
The petrus bones were removed, powdered under ultraviolet sterilized conditions and processed through silica based extraction in a clean room where every surface was decontaminated and every instrument swabbed before use. Of the more than 100 sets of remains, 64 yielded genetic material of sufficient quality for full genome analysis.
Radioarbon dating placed the burials between 600 and 1100 common era, spanning five centuries at the height of Chichinita's political dominance over the northern Maya lands.
The results stopped the team. Every single individual was male. Not one was female. Barera said after the study's publication in Nature in June 2024 that they kept rerunning the tests because they could not believe that all of them were male. DNA says otherwise and it said so 64 times without exception.
Fertility offerings in Misoamerican archaeology had always been associated with female victims. The Cholun held no fertility offering. The assumption built from Spanish colonial accounts carried forward by four centuries of repetition had rested on the wrong bones in the wrong chamber filtered through the wrong lens. What the Cholton held was something the written record had never described.
Among the 64 genomes, at least a quarter of the boys were closely related to at least one other child buried in the same chamber, brothers, cousins, and among them two pairs of identical twins. That finding pointed directly into the deepest layer of Maya religious cosmology.
The Popul Vu, the Kichche Maya creation narrative is based on oral traditions that predate the written manuscript by centuries and is confirmed by painted ceramic vessels from as early as 600 CE.
It centers on the hero twins, Hunapu and Exbalanc. Born from the severed head of their father, the maze god, the twins descend into Chibalba, the Maya underworld, and face a series of trials set by the lords of death. They are killed. Their bones are ground up and thrown into a river. They transform, resurrect, and return, outsmarting the gods of death by allowing themselves to be destroyed and coming back. In the end, they ascend to become the sun and the moon. The story is not a metaphor within Maya cosmological belief. It is a description of how the universe works, the cycle of death and rebirth that sustains the world, enacted and reenacted through ritual.
Subterranean structures like the Chultton were understood in Maya cosmology as entrances to Ethi.
The pattern emerging from the DNA paired male children selected over generations at regular intervals between 500 and 900 CE and deposited in a chamber connected to a cave below ground suggested a ritual designed not to appease a rain god but to reenact the myth to send twins into the underworld to replicate in human form the sacrifice that the hero twins had performed in the sacred narrative. Barera told reporters after publication that we think the people from Chichanita were trying to symbolically replicate the Mayan mythological stories and the representation of the twin heroes in this ritual burial. Carbon and nitrogen isotope analysis of the bones added another layer. The closely related boys, brothers, and cousins had eaten similar diets throughout their short lives, suggesting they grew up together in the same household, fed from the same sources, raised in proximity before being selected. Most were between 3 and 6 years old when they died. The sacrifices were not opportunistic. They were deliberate, theologically precise, and repeated across five centuries by a civilization that understood death not as an ending, but as a mechanism. The sacrifice of twins, in particular, carried a weight that the colonial Spanish accounts had never captured, not because the Spanish did not observe it, but because the framework through which they interpreted what they saw had no room for it. What looked like the killing of innocents was within the Maya cosmological order something closer to a sacred enactment, a choosing, not a taking.
The DNA also traced a line forward in time. Researchers collected blood samples from modern Maya living near Chichen it in the community of Tixkal Tuyub. The genetic analysis found direct continuity between the ancient children in the Chultton and the living population of the region. The same ancestral lines, centuries compressed into a genome still present in the descendants of the civilization that built the pyramid. The colonial epidemics that followed the Spanish conquest, including the 1545 Coco Lizzley outbreak caused by Salmonella and Rica Paratifi, which killed close to 90% of the indigenous population of what is now Mexico, had not erased that continuity. The Maya genetic legacy survived what the political and religious structures could not. The Cholton has not been fully excavated.
Remains beyond the original 64 sample genomes are still being studied. New ground penetrating radar surveys continue to reveal structures beneath existing buildings, sealed chambers, buried construction phases, contexts that predate what the surface shows.
Each new season of fieldwork adds to a record that the colonial accounts condensed into a single misleading image. The young woman at the edge of the cenote.
The bones say otherwise. They always did. It took ancient DNA to make the bones speak louder than the conquestadors.
The children buried in the Chulon died between the 6th and the 11th century CE.
Inside a chamber their civilization believed connected to the land of the dead. They were chosen for who they were, their sex, their kinship, their relationship to a myth older than the pyramid above them. For 400 years, the story attached to those bones belonged to someone else entirely. Now it belongs to them again. Discovery continues.
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