L-29 - NIPER JEE 2026 | P' Analysis: Chromatography | 360 Series #niper #niper2026

Ajouté : 2026-05-23

[00:00:00]Hello dear students welcome to GDC YouTube channel and today we will discuss our 360 degree series for sniper in which we are going to discuss previous year questions related to the chromatography along with the current questions in 360 manner here. So, we all know that chromatography is a separation technique of complex mixture.

[00:00:22]In chromatography, there is a mobile phase that carries its sample and it interacts with its sample through the stationary phase.

[00:00:30]What happens on that basis? Separation occurs.

[00:00:32]Brother, in chromatography, it is the stationary phase that decides what will be the principle of chromatography? If you accept your mobile phase then it is fixed brother. It will either be liquid or gas. The day when the mobile phase becomes gas is called gas chromatography. The day when it is liquid is called liquid chromatography.

[00:00:48]Okay, right? Now if there is a column in the liquid then liquid column chromatography, HPLC, all these come and if there is no column then they have paper and plate, then it is called paper and TLC. So maximum questions on chromatography are seen in the exam.

[00:01:02]Firstly, the principle of chromatography, secondly, the modes of chromatography and thirdly, the detector of chromatography, that is, which detector do we use in which chromatography? The first question comes here, look carefully. Here it has been asked about the most appropriate technique for separating the SO2 and HCL, brother, you have to separate SO2, brother, this is sulphur dioxide, brother, it is also called the king of gases and this is yours, it is also stored in a grey coloured cylinder like carbon dioxide.

[00:01:32]Now a question came brother, the child got scared sir, I have not studied chromatography till date, which mixture should be separated, there was nothing in the question, a gas has been given son, when gas has been given then gas chromatography will have to be done only, nothing else can be done, it will be gas chromatography, if someone asks the mobile phase in this gas chromatography, the most common one is helium brother, it is the most common one, it has good thermal conductivity, hence it is common, if helium is not available then nitrogen can be taken, there are two types of gas chromatography.

[00:02:03]Gas solid chromatography principal becomes adsorption.

[00:02:07]What is the principle of gas liquid chromatography brother? Partition would have happened. So your answer B is absolutely correct. Moving on to the next question. The purity of a drug can be determined by The property of any drug has to be found out. Purification has to be done.

[00:02:21]Look at purification analysis does three things.

[00:02:23]Purification and separation.

[00:02:26]Identification means your quantitative and qualitative analysis. Ok? And estimation means finding out the amount. That is called Quantity Return Analysis, brother. So your liquid chromatography is MS, brother.

[00:02:38]LCMS. Ok? Or there is GCMS, there is mass, there is HPLC. So I gave three chromatographies.

[00:02:44]Finding out the purity of the drug. The options here are such that they are according to the memory based options. Then what answer will we give brother? Chromatography is available in three options. So what will you plant?

[00:02:57]Suppose I also applied it. So I will ask why is none of yours putting this brother, this is not mentioned here brother, our drug is volatile, if it was written that it is volatile then we would have put this brother, if it was written that our drug is liquid then we would have put this brother, you are understanding it right, and HPLC is also no less than anyone, it can also do the purity, in this option brother, this will also find out our purity, this will also find out, this will also find out the mass, it will find out the molecular weight, so if you can put Accept then C option will go away. As for the rest of the purity, brother, all our three options are no less than each other.

[00:03:32]Because if purity and separation is to be done then there is chromatography.

[00:03:37]You also have liquid chromatography. We also have gas chromatography and HPLC.

[00:03:41]If someone asks him, brother, what is this? So we will say that if two methods are used together then it will be called hypenated techniques. If you want an answer here then you will have to write what is your drug? It is volatile. If the drug is volatile then answer B will be absolutely correct. Your answer will be corrected. Ok? Hard Ionization Detected by Bhai is hard ionization technique. If we look at the question, this question is basically about mass.

[00:04:08]Ok? It's hard ionization, brother. MALDI is soft ionization.

[00:04:12]For hard ionization, we have GCMS. Because the ionized sample present in the mass is generally in vapor state. So we will go towards GC. GCMS will be ours. We will also call this our hyphenated techniques brother. Ok? Hyped because this maldi is soft. The problem with LCMS is that it remains liquid here. You have vapor there. So this will not be a little bit FT N MR either.

[00:04:39]Our GCMS will be the absolutely correct answer.

[00:04:41]What will come under hard deionization?

[00:04:43]GCMS will come. Now comes Carrier Gas Not Use in the GC. Brother, the carrier gas used in gas chromatography is helium at the top position, then brother there is nitrogen, we also have argon, okay, carbon dioxide, okay, hydrogen and methane too.

[00:05:00]What is the problem with hydrogen and methane, it is inflammable, so we avoid it, okay, so helium is the most common, we cannot use oxygen, brother, keep this in mind, now here he is asking which one should not be used, okay, so look, here you can use hydrogen, but there is a little risk of it being inflammable, but you can use it and it also gives good efficiency, so you cannot use this, no, helium is the boss, brother, it is used the most, so you cannot use this also, what is left, we do not use chlorine or oxygen, generally your answer C will be correct here brother because we know which ones we use, we know which ones we use and which ones we do not use, so according to that, our answer will be C Carrier Gas Uses in the Gas Chromatography What is the carrier gas of gas chromatography brother? Look, I have not read it. Didn't read Oxygen. You can use carbon dioxide. But as long as helium is there, we will not pay any attention to anyone else. Helium is the most common and has good thermal conductivity. So here your answer will be corrected by us.

[00:06:06]Whatever answer you give will be considered correct. Our answer will be correct.

[00:06:10]Method for Volatile Oil Detection.

[00:06:13]Now the same story happened again. Someone is flying, if you want to catch a flying bird then HPLC will not be able to catch it. Ok? Neither will he be able to hold your paper nor your column.

[00:06:22]Your GC will catch the flying bird. Brother, for volatile nature, we will have gas chromatography.

[00:06:30]What will happen brother? Gas chromatography will be corrected. Brother your residual solvent is detected by.

[00:06:37]Now detect the solvent brother.

[00:06:38]HPLC, TLC, Paper Chromatography, GC.

[00:06:44]You can use GC to detect residual solvent. Ok? Now you can use GC for the residual solvent. Ok? But then the same story, brother, how should the solvent be? Must be volatile. Ok? So if your volatile is a solvent, you can use GC for it. GC which separates compounds on their boiling point. Brother, volatile nature is necessary. We cannot use gas without it.

[00:07:08]Your gas brother, it is basically about the identity and concentration of the residual solvent, but where is the story, son, it should be volatile, see it is written that it will be volatile, so you will do it, okay, a question came about such a drug, there I told you brother, volatile will have to be written, only then you will be able to do it, if you are asking about direct residual solvent, then you are giving GC answer, but rest of the chromatography can also be done, for that work it is very important to be volatile here brother, which of the following chromatographic technique is used for the separation of amino acids. The question came many times and also in the jeep. So for this your TLC, in TLC we know there is Ninhydren brother, ours is distance trouble by the solvent and distance trouble by the solvent. So for amino acids, which of the following chromatographic techniques is used for the separation of amino acids. For amino acids you can use TLC and also do ion exchange my brother. Ok? Look at the ions, why are we discussing it here, brother, we have to learn the right thing, so we can do ion exchange, we can do TLC, we can do both, both can be done, but gas chromatography cannot be done for amino acids because brother, if your thermal temperature increases then there can be a problem, so here you can do ion exchange and you can also do TLC, what happens when there are options, there are other options in that, so what happens because of this, we can do these, principle of gas chromatography.

[00:08:37]Brother, there are two principles of gas chromatography. Ok? He has not said anything. There are two types of GC, brother. One is gas-solid chromatography.

[00:08:44]One is our gas liquid chromatography.

[00:08:46]We are trapped in a confused crisis. Brother, should I install adsorption or partition?

[00:08:51]What answer should I give? Should we apply adsorption answer or should we apply partition brother? Brother, now if we apply adsorption answer then partition will be angry. In such a case we have to go for partition. When nothing is given, whether he asks you the principle of LC or the principle of HPLC. But they all have principals. Adsorption, partition, affinity, ion exchange are all there.

[00:09:13]So in such cases, if there is no mention of stationary phase, then what should be the answer? If there is no mention of stationary phase then what answer should we put? You have to install the answer partition.

[00:09:30]Whenever any point of the stationary phase is not discussed there, then what answer should we give? Partition has to be installed. What do you want to put brother? We have to install the answer partition. Take good care of this thing. Now comes the matter of sharpness of peak in GC is due to.

[00:09:44]Brother, the better the peak you want, the better the work you will get. Increase efficiency. So look, the sharpness of peak that we need in our GC is good so that proper separation takes place. So there are two or three things that happen there. Brother, we have two options in this also. If you talk about normal criminography.

[00:10:02]What is the number of theoretically plate? It is L/dp.

[00:10:06]The more you increase your marks, the better will be the result brother. This is also correct.

[00:10:12]And what is written here can happen even if you control the temperature. Ok?

[00:10:18]So suppose you have to choose only one, then son, option A will be considered the most correct because your main number is our theoretical flat number. The temperature has to be optimized, neither too low nor too high. Ok? So here whatever answer you give will be considered most correct.

[00:10:38]Ok? You have to keep this in mind.

[00:10:40]Lower temperatures increase the retention time. The interaction that takes place between you and your brother starts getting better.

[00:10:45]It is possible that by decreasing the initial column temperature one can get a sharper pick.

[00:10:50]But this point is for personal gas chromatography. But that's the whole point of chromatography. The above is also being followed for gas, so for everyone. So in this A is also correct and D is also correct. Both options are the same. It is an exam question, so we have not changed it much. There must be something in the options there? It can be up or down. So here option A will also be correct and our option D will also be correct. Ok? Now let's talk about which chromatography is GLC, it will be made, there is no problem for everyone.

[00:11:20]Suppose if in this question you see GLC is asked then no doubt brother, partition will be installed.

[00:11:25]Suppose in this question he had written, tell the principle of gas chromatography. Then we have given you both, so we would have applied both. Now he has given both and if he has written GLC then this will be the answer. If you have written GSC then this will be the answer. If you don't want to suck one of the two, then we will do the partition.

[00:11:45]Now brother, suppose they had given both and if only GC was written for us, then what would we have written for Gas Chromatography, then we could have written C on the answer. So there is no problem right now. A is the answer.

[00:11:57]He didn't bother us much. He said, brother, give a straight answer and move ahead.

[00:12:02]Tailing in HPLC observed due to.

[00:12:04]Ok? Equal Affinity Between the Analyte and Stationary Fuzz. Brother telling happens when our soulmates become more than just stationery fuzz.

[00:12:14]His concentration keeps waning little by little throughout the day.

[00:12:16]Equal Affinity Between the Annulled and Stationary Fuzz. More Affinity Between Analyzed. This friendship is growing, son. There is danger here. Friendship will grow. The graph will look like this. There will be no friendship. The common pick will come. You will increase your friendship. It will keep coming out throughout the day.

[00:12:32]This will be the tailing off peak. Ok? So this is More Affinity.

[00:12:36]Universal will come in less affinity. Charioteer can also come in No Affinity.

[00:12:39]Ok? So separation will not make any sense. The more friendship grows here, with whom? From Stationery Face.

[00:12:46]The longer the retention time will be. The illusion rate will be slow and you may have tailing off peaks.

[00:12:52]What can happen to us?

[00:12:54]We can see the tailing of the peak. Take good care brother. Now comes the question brother, which is the universal detector? What are you asking, sir? Okay, ask about anyone, friend, if you are asking about anyone then we understand your feelings, if you are asking about gas chromatography then there is a catherometer brother, it is also called thermal conductivity and this is what is called universal detector sir, it finds out organic compounds including the halogen, but the best one for halogen is our electron capture detector, okay, so you are asking about refractive index, is it universal brother, that is absolutely right, GC's is universal, you understand concentration, see, GC's detector sir, revise it well, you guys come to the exam. Look, the mass flow guy lives in GC. Brother, you will have to remain fit in the mass flow.

[00:13:38]How will I have to live?

[00:13:40]Fit in flame is an ionization detractor. The most common one which is widely used is ours. Ok? Flame ionizes which contains hydrogen flame. Ions are formed and the current changes. Let us take a look at that.

[00:13:51]In this, thermospecific nitrogen phosphorus and flame photometric are our mass flow ones.

[00:13:58]Sir, our PET comes in the concentration system.

[00:14:00]Thermal conductivity catherometer Wheatstone bridge universal electron capture is best for halogens bhaiya. Ever asked the question which detector for halogen? So do n't put any answer other than ECD. If ever asked, brother, can you do TCD for halogen?

[00:14:19]But what is the best? This is and this photo ionization this happens. When it comes to HPLC detectors, bulk properties are the most common term.

[00:14:27]RCB runs there. Refractive indexes are bulk properties of conductivity. Ok? So if you have asked us about Universal Detector then Cathorometer will be your correct answer. Cathodemeter, Bolometer, Glob Cell, IR refractive index and conductivity are the methods of HPLC and are bulk property detection. So this is not a problem at all. I am asking here, brother, if you ask about HPLC, which one is commonly used? Will put a refactory index. If you have asked about the universal of GC, then for the word universal that you have written, catherometer is your correct answer. The name of chromatography is not mentioned there for GC, brother. Now the question is Detector. Now son, everything including detective is there in that option. Whose detector are you asking?

[00:15:09]Tell me this. Now comes the use of the photo diode array detector. HPLC uses mass, UB, or atomic absorption. So, you can use this question in HPLC also, brother.

[00:15:21]Photodiode Arride Detector. Ok? You can use it in HPLC brother.

[00:15:26]Correct. Ok? You can do it in this also, you can do it in this also. It can happen in all three.

[00:15:30]Our photodiode detector is for HPLC but it can also be used in atomic absorption and UV. Got it, right? So what do you have to do with options here? These will have to be removed, brother. This is the main one.

[00:15:46]You will have to write it instead. Suppose NMR has to be written here and IR has to be written here. Then we will install HPLC. What will you plant? We will put the HPLC answer.

[00:15:56]Detector Use in the HPLC. Look at this straight, it is written which detector is used in HPLC? Which detector do we use in HPLC? It is written straight.

[00:16:07]Now look, the bolometer in this is saying, Sir Bhaiya, we are from IR. We don't have to.

[00:16:12]Ours is the Refactory Index. Globular cell belongs to IR. The one in the photo is of our UP bill.

[00:16:17]So what will be the answer now? Our answer is absolutely correct brother. Your reflective index will become completely correct. It is temperature dependent. And what does it come in?

[00:16:27]Comes in bulk property. Which one does it come in?

[00:16:29]Comes in bulk property. It is temperature dependent and what does it fall into?

[00:16:33]Comes in bulk property. take care brother. Ok? Now comes the next question.

[00:16:38]Indicate the HPLC detector that is most sensitive to changes in temperature. What I was talking about just now is the same question brother.

[00:16:44]One of our refractive index detectors is temperature sensitive, brother. I have asked you the same thing. Look, PDA will not come.

[00:16:50]Photo diyo will not scare you brother. Ok? This will not be yours. This can also be used.

[00:16:55]This will come under solute property. Have to be careful.

[00:17:00]This is the answer with the Reflective Index Bulb property. This will come under solute property. This will also come under solute property. What will be your answer?

[00:17:07]B. Our answer will be correct. B Answer will be considered correct.

[00:17:13]Your answer will be perfectly correct. B.

[00:17:16]Correct your answer. The next question is which of the following HPLC detectors is not a solid property detector?

[00:17:23]Brother has come again. Look, solid property means personal car son and if you are travelling in public vehicles then it is called bulk property. In that RCB comes refractive index and conductivity. So he is asking which of these is not a solute property. UV Bijal Bhaiya is personal property. Lambda Max Personal will come to them. The photo diode is personal, brother. Ok? They can also use us. I also had a question in HPLC.

[00:17:47]But these photos will also go into UB Visual. Would have also gone into atomic absorption.

[00:17:51]Rosence wala also all this solid property is Detector. Solid Property Detector can be anything. A chemical test may be done. It can be any spectroscopic method. But RCB should be careful in bulk properties. So this refractive index, which is not a solid property? The refactored index is our bulk property. So your option D will be correct.

[00:18:12]Now comes brother, what is normal phase HPLC? This is a very good question. This is coming brother. Hey, these modes are used in any chromatography. Brother, will you go to the station? Ok? Normally, if you go to the station, you will get water. So the stationary phase in the normal phase is polar.

[00:18:29]So what is mobile phase brother? It is non-polar.

[00:18:32]If someone says the opposite of this, in reverse phase our stationary phase is non-polar while the mobile phase is polar.

[00:18:40]Another question they ask a lot. Who will come first? Who will come later? In this, the son who will be like the stationary phase will come later.

[00:18:47]Whatever the mobile phase is, it will come first.

[00:18:49]Ok? So brother, if you look at this, polar comes first. In this, non-polar ones separate first. So, I have not asked all this. What was asked in the normal phase?

[00:18:57]Mobile phase. The mobile phase will be non-polar brother.

[00:19:00]Ok? So there is non polar mobile phase. The stationary phase is its polar. So whatever our answer will be, your answer will also be absolutely correct.

[00:19:08]Remember it well.

[00:19:10]This can be asked in any chromatography.

[00:19:12]Where is the preparatory column used brother?

[00:19:14]Used in gas chromatography. And when we have to purify something, we need a pure sample, then what do we do for it? Let's put a preparatory column.

[00:19:23]Separate the compound of high volatile nature, Obtaining mixture of compound, Separating two miscible compounds, Obtaining the pure compound. It is written straight, brother. The additional column of chromatography is gas chromatography. If we want the sample in pure form then what should we take brother? Let's put the preparatory column here.

[00:19:43]P for Preparatory P for our becomes pure. Take good care.

[00:19:47]Now comes the length of a column is double then the efficiency increases by a factor. Let's see what happens to us? What is the problem brother, as these numbers increase the efficiency will increase.

[00:19:58]What did you do to the length? You have doubled it, sir. This n is directly proportional to the length brother. If you double it, it will become double.

[00:20:06]What happened next? Ok? If you double the length, it will double. Brother, what's the problem brother? If you double the length, it will double.

[00:20:14]Will ask for efficiency. What is n equal to? It is l/htp. The more you increase it, the more it will increase. If you double it, it will double. Ok? Whatever answer we give here, our B option will be absolutely correct. There is no numerical. This is a small question. We have to remember this brother. If you did not do anything here? l/hdp done. So simply brother, if you double this, it will become double.

[00:20:39]Okay, right? Your answer will be correct.

[00:20:41]Now comes which of the following statements is correct about open column chromatography.

[00:20:46]Ok? In open column chromatography the term open means the open setup. okay brother? Let's look ahead. Active sites present in the column are polar in nature. Look, if you are keeping polar nature then it means you are carrying polar in your stationary phase. So brother, this is your normal face.

[00:21:04]We can accept this also. The mode of separation is adsorption and the separation is based on the polarity of the substance. This can also happen.

[00:21:11]So in this question brother there is no option which is for open liquid column chromatography? You may have given it wrong.

[00:21:18]Brother, all your sentences are correct. There is no such thing as wrong. Brother, a general thing has been said and all our sentences are absolutely correct.

[00:21:28]What is there no sentence here? It is not wrong. Everything is fine. We will put all the answers. What will you put brother? I will put all the answers. Now comes the next column, chromatography is your brother. Ok? Which of the following adsorbent has lowest adsorption property. Silica alumina is the most popular, brother. Whose is the lowest?

[00:21:47]So brother, remember the lowest one is cellulose or inulin, starch, what happens to those carbohydrates? Low is the best. Then our answer will be simple. Which has the lowest adsorbent power? It is in carbohydrates.

[00:22:01]See who the good ones are? Look, I can see this brother. Silica is the best and is the strongest. Alumina is strong brother. Charcoal is strong.

[00:22:10]Magnesia is strong. Ok? So you have to remember this well. This scam is a strong scam brother.

[00:22:18]This is where the scam happens. We say brother, there has been a scam. So this is silica gel, alumina, charcoal and the magnesia here are strong.

[00:22:27]Scum is strong. Ok? Keep this in mind.

[00:22:30]And that's how I read them.

[00:22:32]Carbohydrates sucrose, starch inoline, cellulose talc and sodium What are these? Comes in a week.

[00:22:39]These people come in the medium. Calcium carbonate, calcium phosphonate, magnesium carbonate. Which of ours do they come in, brother? Come to our week. So, we will remember this a little bit. Remember the strong ones and the weak ones. Now comes brother Vi of the following correct increasing order of illuminating power of the solvent in TLC? A little question like this that the one who has the highest illusion power is that of the Padin.

[00:23:01]So your answer is Padin.

[00:23:04]Benzene has the lowest. So benzene is given in the last.

[00:23:06]Padin is given at the top. So brother, whatever your answer will be, our D option will be correct.

[00:23:11]Padin is on top. Benzene is in last. Ok? Padin is on top and where is his benzene? It is in the last. Benzene is in its last position. Benzene is our last one.

[00:23:22]Now comes the next question here. Solvent Use in Reverse Phase Chromatography. Then it's the same story brother. What happens to itself in the reverse phase?

[00:23:31]Solvent means mobile phase is asking you. Brother, what is the stationary phase that occurs in the reverse phase? It is non-polar.

[00:23:39]Ok? The mobile phase is polar.

[00:23:41]Now find the polar mobile phase in it. It is okay if you feel lonely. This can also happen.

[00:23:46]But it would be better if you take the mixture. Brother, what is the problem? It would be even better if you take the mixture.

[00:23:51]Our mobile face will be good and it is also polar. Ok? We know that there is confusion in the methods of column development. In illusion analysis, gradient illusion is considered good. The composition changes.

[00:24:03]And what is the composition in isocratic? The beans remain. So here, do not put your B option in haste. Read B also brother. Ok? Your A is correct but what is correct from A, your B is reverse phase TLC drug with low polarity, can B be your illiterate brother, see it is reverse phase TLC, write carefully what is the stationary phase brother, it is non polar in it, okay and what is your mobile phase, it is polar, what do you have to do with the drug, okay, if you want to extract it first then you will have to make it like the mobile phase, if it becomes like the mobile phase then it will come first, now how will you make it like the mobile phase, brother, you will have to increase its polarity, brother, okay, increasing the polarity of the mobile phase, increasing the polarity of the mobile phase, okay, decreasing the polarity of the mobile phase, brother, make the mobile phase like that, your work will be done, okay, so now see brother, either make your compound or make the mobile phase, so this is what you have, brother, our mobile phase is low polarity, make it like the mobile phase, if you make it like the mobile phase then it will come out, brother, increasing the polarity of the mobile phase, you will get the mobile phase And just match the sample, it will come first and the stationary phase and if the sample is matched, it will come later. It 's a simple story. You don't have to remember anything in this. I do n't remember anything brother, yours is about to be beaten.

[00:25:23]Called the mobile phase low.

[00:25:26]This could be an option. Now comes which of the following is the principle of the gel permeation chromatography? Brother, filtration happens directly here. Let's take a Sephadex column.

[00:25:35]If they take over Joe Dakins column.

[00:25:37]Ok? Here, the large molecular weight ones come first and the smaller ones get trapped inside. So they come out later.

[00:25:45]Separation takes place on this basis. So it works according to the size. The separation that takes place in this is done according to the size. Keep in mind here, suppose someone has 4 oz Dalton.

[00:25:55]Ok? And one is of 30000 Daltons and one is of 10,000 Daltons. Who will ask first? So don't give an answer like filtration.

[00:26:05]This will come first because it will not be embedded inside the polymer. Ok? This will come first.

[00:26:10]Whatever these things are, they will come later. This little one will come out last because what will it become inside the polymer? Your intp is done. So keep this in mind brother, we know that in affinity chromatography there is Southern blotting. It is of DNA. Northern blotting is done of RNA. Western blotting is for proteins. Come to Affinity. Ok? So here your answer C will be correct brother. C Answer is correct. Next is the size exclusion technique used for the determination of the molecular weight. Tell me, you just saw what you have to put inside it for molecular weight? Applying N lipid will not work. Ok?

[00:26:48]No enzyme solvent, you will have to apply polymer brother. For this we use Dexin.

[00:26:53]Ok? Our own dexin is used. Let's take Dexin, my brother. We also include agarose in this. Ok?

[00:27:02]Also take polyacrylamide gel. So these remain with us. What do I have to put in, brother? Polymer will have to be applied. We will use polymer in this. Now comes the common binder used in the silica gel.

[00:27:14]Son, I am asking the full form of ji. Ji ka Apna is written as Silica Gel Ji.

[00:27:18]What is written in TLC? Silica gel g. So what happens? G stands for Gypsum, sir. And we know gypsum as calcium sulphate 2H2. What is that gypsum and calcium sulphate half H2O called? It is called plaster of Paris. Ok?

[00:27:35]So all this is fine.

[00:27:37]Calcium sulphate will come here and it will be fine. And if someone asks, silica gel is known for its acidic compound. And alumina is for basic compounds. Take care to apply adsorbent material on the TLC plate properly.

[00:27:52]What do we do? Coat it on your TLC plate as an adsorbent material. What is done with it? It is coated on the TLC plate.

[00:28:03]What do we do with it in the form of adsorbent material? We coat it. So, my answer is absolutely correct. Which of the following chromatography is based on the molecular size? It is a very simple question.

[00:28:12]HPLC says brother, its performance is liquid chromatography. Your mobile page goes through its pump. The effect of gravity does not come in 5000 to 6000 psi or more, 5000 plus is fine. The principle of TLC is its main adsorption. GC, we know well, will come with gel permeation chromatography. If we have to separate according to the size, then what will be the gel permeation chromatography?

[00:28:37]If we have to separate according to the size, what will be the gel permeation chromatography? It will be ours.

[00:28:42]Remember it well. If we have to separate according to the size, then the gel permeation will be ours. Let us discuss further which of the The following adsorbents are used in chromatography. Brother, you have said a very important thing.

[00:28:54]Adsorbent is asking.

[00:28:56]Look, silicone oil is used in chromatography.

[00:28:58]Absolutely son, this is the liquid stationary phase. Ok? So this will not be the answer. Ok? If we talk about Carbomarg, it is also used. This is the liquid stationary phase. It's all liquid.

[00:29:09]Silica gel will come here. The most common adsorbent is silica gel.

[00:29:14]In Strong, I told you that there is a scam. Contains silica gel.

[00:29:18]You have alumina. Ok? This is done brother, this is your charcoal. Contains magnesia. So these and the weak ones contain carbohydrates.

[00:29:25]We also have a little bit of talc and sodium carbonate. You have to be careful brother.

[00:29:29]Ok? So we discussed all these questions related to chromatography. After this we will meet in the next part. Keep studying well. Keep reading great. Look, the questions in Sniper are memory based, son. Many times we think, Sir, how is this happening?

[00:29:44]Three options are correct in this question. There are two getting it right. Hey brother, those are options.

[00:29:49]Those options will be available to you soon. There you will get only one answer. Eliminate three. Do you understand? So what does our discussion mean brother? This is not ours, brother. Suppose your option A is correct. Suppose B is also correct. So I should say no because A is written there. No brother, whatever is there is there brother. If your two are correct then two are correct. If there is some mistake in the question then they will tell you brother that this thing is wrong in the question. Do you understand? So this is how we have to work. Ok, see you in the next part brother.