CHM256 Video Presentation DETERMINATION OF VITAMIN C CONTENT IN ORANGE JUICE USING IODOMETRIC TITRAT

[00:00:14]H so yummy.

[00:00:16]>> Wow. Orange juice.

[00:00:17]>> Yeah.

[00:00:18]>> Do you know orange juice is one of the most popular beverages consumed around the world? It is known very rich in vitamin C and providing many health benefits.

[00:00:28]>> Oh, really? But have you ever wondered how much vitamin C is determined in our orange juice?

[00:00:35]>> Can we determine its concentration accurately?

[00:00:38]>> The answer is yes using an analytical method known as iodometric titration.

[00:00:43]>> Today we will determine the vitamin C contain in orange juice by using iodometric titration.

[00:00:52]>> Why do people always say orange juice is rich in vitamin C? Because vitamin C is an essential nutrient that our body needs.

[00:01:01]>> It functions as an antioxidant and help protects our body from harmful free radicals. Vitamin C also plays an important role in collagen synthesis while healing iron absorption and strengthening our immune system.

[00:01:17]>> Since our body cannot produce vitamin C naturally, we must obtain it from food sources. Among the saucers, orange juice is one of the most popular and richest sources of vitamin C.

[00:01:32]>> Therefore, determine the vitamin C content in orange juice is important to evaluate its nutritional quality.

[00:01:39]>> So, how can we determine the concentration of vitamin C in orange juice? Let's find out in this experiment.

[00:01:48]So, what are we trying to achieve in this experiment? First we want to determine the concentration of vitamin C in orange juice.

[00:01:57]>> Second we apply iodatic nutrition as a quantitative analytical method.

[00:02:02]>> And finally we calculate vitamin C content in dilute and original orange sample. So let's see how the methods work.

[00:02:09]>> So now we will talk about application of vitamin C determination. The determination of vitamin C content has many important applications in our daily life. It is widely used in food quality control to ensure that the food products contain the expected amount of nutrients. It is also important for nutritional labeling allowing consumers to know the nutritional value of beverages or the products. Besides that, vitamin C analysis can be used to monitor nutrient degradation during storage and compare vitamin C among different fruit juices. In addition, pharmaceutical and supplement industries use this analysis to verify the amount of vitamin C present in their products.

[00:02:58]The experimental data used in this presentation were obtained from a laboratory demonstration video entitled determination of vitamin C in orange juice using iodometric titration. The video demonstrated the preparation of solutions, titration procedures, observations, calculations, and final results. All the data and calculations presented in this study are based on the experimental results obtained from this laboratory demonstration.

[00:03:32]>> Now let us discuss the principle of the experiment. This experiment is based on idomatic titration which is a type of redux titration under volutric analysis.

[00:03:44]In this reaction, vitamin C acts as a reducing agent. It reacts with iodine and converts iodine into iodide ions while vitamin C itself is iodized to dehydroc.

[00:04:00]After all vitamin C has reacted, the remaining iodine is titrated using sodium sulfate solution. Therefore, the amount of iodine consumed is directly proportional to the concentration of vitamin C present in the orange juice sample.

[00:04:19]So the chemical that we use in this experiment were sold dioulfate potassium dromate iodine potassium iodine starch indicator dist water and fresh orange juice. Meanwhile the apparatus used include the bureate pipette conical flatic fl and cost clothes.

[00:04:44]Before carrying out the titration, several solution needed to be prepared.

[00:04:49]First, sodium teosulfate solution was prepared by dissolving 0.625 g of sodium tulfate in distilled water and making up the volume to 250 ml. Next, potassium dicromate solution was prepared by dissolving 0.049 g in 100 ml of this water. This solution act as a primary standard during the standardization process. After that, iodine solution was prepared by dissolving 0.3 grams of iodine together with one gram of potassium iodide in this water and making up the volume 200 ml. Potassium iodide was added to increase the solubility of iodine in water. Finally, starch indicator was prepared by mixing starch with warm water, boiling it and allowing it to cool before use. After preparing all the required solutions, the orange juice sample was prepared. Fresh orange was squeezed and filtered through a clean cloth to remove pop and solid particles.

[00:05:54]Then 10 mm of orange juice was transferred into a 100 ml volutric flask. Distilled water was added until the calibration mark was reached producing a 10fold diluted sample. The juice was prepared immediately before analysis to minimize the oxidation of vitamin C due to exposure to air.

[00:06:20]>> Next we move to the titration procedure.

[00:06:23]First the borate was rinsed and filled with sodium sulfate solution. Then 10 mm of dilated orange juice and 10 mm of iodine solution were transferred into a conical flask. The mixture was titrated with sodium tulfate while swirling continuously. Initially the solution appeared brown due to the presence of iodine.

[00:06:46]As the titration proceed the color gradually changed to pale yellow. At this stage, several drop of starch indicator were added causing the solution to turn dark blue purple because of the formation of the starch iodine complex. Finally, the titration was continued carefully until the blue purple color disappeared completely indicating the end point of the titration. The final B reading recorded was 23.4 mm.

[00:07:22]Beside the sample titration, a blank titration was also performed. In this case, only iodine solution was used without adding oranges. The purpose of the plant titration is to determine the total amount of iodine initially present in this solution. The titration procedure was repeat using sodium tulfate solution and the final bure reading obtained was 25 ml. By comparing the blank and sample tations, we can determine the amount of iodine that react with v vitamin C.

[00:07:58]>> Okay. Now let's talk about results in this experiment. Firstly, the blank titration required 25 ml of sodium the sulfate. Meanwhile, the orange juice uh sample required 23.4 milll. The difference between these two values was 1.6 ml. This different represents the amount of iodine consumed by vitamin C present in the orange juice sample.

[00:08:24]Starch act as an indicator by forming a dark blue purple complex with iodine. As sodium sulfate reacts with iodine, the iodine concentration gradually decreases. The end point is reached when all iotin has been consumed and the blue purple color disappears completely.

[00:08:41]Therefore, starch play as important role in detecting the end point accurately during the titration process. Based on the titration results, the marity of vitamin C in the diluted orange juice was calculated as 8.16 * 10 power of -4 m. The vitamin C content was found to be 143.77 mg per liter which is equivalent to 143.77 ppm.

[00:09:16]After correcting for the 10fold dilution the marity of the original orange juice was calculated as 8.16 * 10^ of -3 m.

[00:09:28]Then the vitamin C contain in the original orange juice was approximately 1.43 g per liter.

[00:09:39]>> Based on the result, the blank titration required 25 ml of sodium tulfate while the orange juice sample required 23.4 ml. The different of 1.6 ml indicates that iodine had reacted with the vitamin C in the orange juice before the titration. This agrees with the principle of idomatic titration where a higher vitamin C content consume more iodine leaving less iodine to react with sodium tissulate. Possible source of error include parallel error when reading the borates. Difficulty in identifying the end point and oxidation of vitamin C due to the exposure to air.

[00:10:20]To improve the accuracy of the experiment, the titration should be repeated. The juice should be prepared immediately before analysis and fresh starch indicator should be used.

[00:10:33]In conclusion, the vitamin C contain in orange juice was successfully determined using iodometric titration. The daily sample contain 143.5 mg per liter for vitamin C while the orange juice contain approximately 1.43 gram per liter. This experiment demonstrate that iodometric titration is an effective and reliable quantitative analytical method for determine vitamin C content in fruit juice. Therefore, all objective of this experiment were successful.

[00:11:13]in the pool. I smell like a mini bar.