CHM256 PRESENTATION GROUP 4 A4AS1204_4 - APPLICATION OF CHROMATOGRAPHY IN LIPSTICK ANALYSIS

[00:00:04][music] >> Have you ever wondered how a lipstick has the same vibrant color every time you buy it?

[00:00:10]Behind the scenes, cosmetic [music] manufacturers perform quality control tests to ensure each product meets safety [music] and quality standards.

[00:00:19]One of the most important techniques used is chromatography.

[00:00:23]Today, >> [music] >> we'll see how chromatography helps analyze lipstick pigments.

[00:00:32]>> Good morning, Glam Beauty Cosmetics.

[00:00:34]This is Mother speaking. How may I help you?

[00:00:38]>> Hello. I recently bought a lipstick in the shade of cherry rose, but the color is different from the one I bought before.

[00:00:45]>> Oh, I'm so sorry to hear that. Could you explain the difference?

[00:00:49]>> Yes, one lipstick is darker, while the other is lighter, even though they are the same shade name. I'm concerned about the product quality.

[00:00:58]>> Thank you for informing us. We take quality control seriously and will investigate this issue immediately.

[00:01:05]>> Thank [snorts] you. I hope the product is safe to use.

[00:01:08]>> You're welcome. Have a nice day.

[00:01:26]>> [music] >> Good morning, everyone. Welcome to Glam Beauty Cosmetics Quality Control Laboratory. Today, our [music] team will demonstrate how chromatography is used in analysis of lipstick pigments to ensure product quality [music] and safety.

[00:01:48]>> Before we begin our investigation, [music] let's understand the field behind this analysis. Analytical chemistry is the branch of chemistry [music] that focuses on identifying and quantifying chemical substances. It plays an important role in various industries such as food, pharmaceuticals, cosmetics, and environmental analysis.

[00:02:11]One of the most important techniques used in analytical chemistry is chromatography, which allows scientists to separate components >> [music] >> in the mixture.

[00:02:22]To explain chromatography in more detail, let's hear from our analytical chemist, Alicia, who is joining us remotely. [music] >> Hello everyone. I am Alicia, the analytical chemist at Clé de Peau Beauté Cosmetics.

[00:02:36]Chromatography is [music] a separation technique that separates substances based on their different affinities towards two phases, the stationary phase and the mobile phase.

[00:02:47]>> [music] >> There are several types of chromatography, including paper chromatography, thin layer chromatography [music] or TLC, gas chromatography, and liquid chromatography. [music] In our investigation today, we will use paper chromatography to analyze lipstick [music] pigments.

[00:03:06]Now, let's discuss why lipstick analysis is important. Lipstick contains different substances such as dyes, pigments, oils, and waxes.

[00:03:21]Analysis of lipstick is important for several reasons.

[00:03:25]First, it helps manufacturers maintain product quality and ensure consistent color. Second, [music] it can help identify counterfeit cosmetic products.

[00:03:35]Third, it can be used in forensic investigation [music] to compare lipstick samples found at a scene. Finally, it helps ensure that cosmetic products are safe [music] for consumers by detecting harmful substances.

[00:03:50]Okay, what is principle chromatography?

[00:03:53]>> [music] >> Principle of chromatography is based on the separation. Different pigments dissolve in differently [music] in the solvent and therefore they travel at different speeds along the chromatography paper.

[00:04:06]In this experiment, [music] the chromatography paper is acts as the stationary phase while the solvent acts as the [music] mobile phase.

[00:04:16]As separation occurs, different colored bands are formed on the paper. [music] >> Lipstick chromatography involving two phases, which is stationary [music] phase and mobile phase. In studying lipstick chromatography, the stationary phase is the chromatography [music] paper which remain fixed while sample is processed.

[00:04:39]While mobile phase is the solvent that [music] travels through the stationary phase carrying the dissolved pigment with.

[00:04:47]In advanced analytical settings, scientists [music] may use uh specialized extraction techniques such as magnetic solid phase extraction to isolate specific lipstick components before [music] they are analyzed through the column.

[00:05:06]>> To perform this analysis, we use lipstick samples, chromatography paper, ethanol or [music] acetone as the solvent, a capillary tube, beaker, pencil, and ruler. [music] These materials allow us to carry out paper chromatography efficiently and safely.

[00:05:25]>> First, [music] a baseline is drawn on the chromatography paper using a pencil.

[00:05:30]The lipstick sample [music] is then applied onto the line using a capillary tube.

[00:05:35]Next, solvent is poured into a beaker and the chromatography [music] paper is carefully placed inside ensuring that the sample spot remains above the solvent level.

[00:05:45]>> As the solvent moves [music] upward through capillary action, the pigments separate. Finally, [music] the paper is removed, dried, and the separated pigment bands are observed.

[00:06:08]>> [music] >> Let's calculate our RF value. Our RF value is determined by [music] dividing the distance traveled by the pigment by the distance traveled by the solvent front.

[00:06:24]For example, [music] if a pigment travels 4.0 cm while the solvent front travels 8.0 cm, [music] the RF value is 0.5.

[00:06:37]Different pigments [music] have a different RF value, which helps in their identification.

[00:06:43]>> The result of lipstick chromatography is [music] a visual chromatogram featuring distinct color bands that serve as a unique chemical fingerprint of the sample. By analyzing these bands, researchers can identify [music] specific red colorants, detect hazardous contaminants like heavy [music] metals, and uncover illegal additives such as sulfonamide antibiotics.

[00:07:07]This chemical profile [music] is essential for forensic investigations, verifying product safety in various markets, and ensuring [music] that lipstick compositions match their labeling.

[00:07:20]>> After the experiment, several color bands may appear such as red, pink, orange, [music] and yellow.

[00:07:27]Each band represents a different pigment in the lipstick. The presence of the multiple bands shows that lipstick contains a mixture [music] of pigments rather than a single color component.

[00:07:38]This demonstrates [music] how chromatography can separate and identify pigments, making it a useful technique for quality control, [music] safety testing, and product analysis.

[00:07:52]>> Chromatography may [music] offer us many advantage because it's simple, inexpensive, fast, and it is easy to perform.

[00:08:01]However, [music] it also has a limitation. It may be less accurate than advanced instruments.

[00:08:08]Some pigment may overlap, [music] and the result can affect by solvent selection.

[00:08:16]>> [music] >> In conclusion, chromatography is an important analytical chemistry technique [music] that allows the separation and identification of lipstick pigments.

[00:08:27]This [music] method is widely used in the cosmetics industry, quality control [music] laboratories, and forensic science to ensure product safety and authenticity.