A protoplast is a plant cell without a cell wall, containing the plasma membrane, cytoplasm, and nucleus, obtained by enzymatic removal of the cell wall using cellulase and pectinase; protoplast fusion is a biotechnology technique where two protoplasts are fused using chemicals like PEG or bioelectrical methods to form hybrid cells that can develop into new hybrid plants through tissue culture, enabling crop improvement and disease resistance.
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Protoplast Fusion Explained in 1 Minute | Botany Shorts.
Added:A protoplast is a plant cell without a cell wall. It contains the plasma membrane, cytoplasm, and nucleus.
Protoplasts are obtained by removing the cell wall using enzymes like cellulase and pectinase.
They are widely used in plant biotechnology and genetic engineering.
Protoplasts help in techniques like protoplast fusion and somatic hybridization.
Protoplast fusion is the process of fusing two protoplasts to form a hybrid cell. It is an important technique used in plant biotechnology.
Fusion can be induced using chemicals like PEG or bioelectrical methods. The fused cell can develop into a new hybrid plant through tissue culture. Protoplast fusion is used for crop improvement, disease resistance, and somatic hybridization.
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