Analysis of Food Dyes in M&M's Using Paper Chromatography

[00:00:00]NHI we are from A4 ES12049 today we presenting about the analysis of food dyes in M&M using paper chromatography under CHM256 and prepare for Dr. membership introduction, theory, real world application, methodology, result, calculation, discussion, conclusion and reference mafi, my ID student 2024 933593.

[00:00:37]I would like to talk about >> of fruit dry as activation fruit dice synetic color addictive are using globally to make food v visual approach maintain color uniforms.

[00:00:54]The regulatory bodies like the FDA and EFSA tightly control which dry can be used. Accurate and separating test drive assure ring fruit safy and complete sense. The M&A case study. Popular candies like M&N use a blend of specific FDNC fruit drops and cosmetic synthetic dry to create their signature shield colors.

[00:01:28]The anatic challenging individual candy shell offer looks like a single color but are actually mixing of a multi primally dry. we need to uh viable way to isolate them.

[00:01:46]Now let's deep into the code theory behind how the separation actually works. Paper ctomography relates on two main components. Uh stationary f and the mobile f. Our stationer f is simply the ctomography paper itself. We are made a tightly wic cellul fiber. it stay complete stay uh the mobile wave wave is our liquid solvent as it sit at the bottom of the tank is naturally clam up the paper to calibrate action draining our sample along with it. So how do the different fruit dry separate is all come down to the chemical t of what be based on ainity mean how strongly a drug is attract to the paper or the liquids.

[00:02:47]drive they they are highly sourn in the sovereign love the mobile path so they travel fast and moves much further up the paper on the the other side uh drive that interact more most strongly with the saloon lo fiber prefer the stational valve so then get held back and move a much stronger distance. There is a different in movement is actually how allow us to see the individual color compartment cleanly separated and my student ID is 20249471 and right now I would like to talk about real world application.

[00:03:39]So the first one is food safety and analytical methods divide by three and then first one is comprehensive review on analytical trends which is broad look at how labs detect restricted or elicit ties globally and then the second one is HLC reference methodology read about the gold standard validations process that industrial lab use for separating complex plan of dice and then the last one is multi-day quantifications for a study tracking the concurrent identifications of 11 synthetic dies including red, yellow and blue under regulatory low guideline. Next quality control industrial applications divide to two which is testing commercial food stuff and industrial additive overviews.

[00:04:24]The first one talking about foundational study exploring high throughput method to extract and analyze dice from commercial products like co candies, drinks and powders. It can be found in the detemination of synthetic dice in selected food staff by HPC with your VD detection. And then the last one is paper chromatography and classroom guide. Also divide to two. First classroom protocol guide. And then the last one is calculating RF values.

[00:04:54]Function of RF values is a comparative look at utilizing paper chromatography alongside modern software tools to isolate RF values. The next one followed by method here are the procedure of an experiment and man on a chromatography paper. First cut chromatography paper into strips and draw a baseline with a pencil. Second one is extract dye from M&M candies using a drop of water and prepare food coloring sample. Next, pour the d solution into the baseline and allow it to dry. followed by prepare 0.1% salt solution as the mobile phase and then suspend the chromatography strips in the solvent with only the bottom edge touching the solutions.

[00:05:40]Moreover, allow the solvent to rise by reactions until it is 0.5 cm from the top of the paper. Then number seven, remove the strips, mark the solvent plants and allow them to dry. And the last one is measure the distance traveled by the solvent and dice box.

[00:05:58]Then calculate the RF values and compare the candy dice with the food coloring standing.

[00:06:04]So here are the pictures that can guide you guys if you guys want to do that experiment.

[00:06:10]Next I will pass this presentation to Lori.

[00:06:16]Now we will move on to the next slide.

[00:06:19]The slide shows the result obtained from the paper chromatography analysis of food dice in M&M's. Based on the chromatoggram image shown, the dice separated into different color spots after the solvent travel through the chromatography paper. This indicates that the M&M coating contains their component that move at different rates.

[00:06:41]The distance traveled by the solvent was measured as 14 cm for all samples. For the red spot, the dye did not move from the origin and record a distance travel of 0 cm, giving an RF value of zero.

[00:06:58]This indicates that the red dye has a stronger attraction toward the stationary phase, which is the chromatography paper, and lower solubility in the solvent. For the yellow spot, the D traveled 8 cm, resulting in an RF value of 0.57.

[00:07:16]This shows that the yellow dye had a moderate interaction with both the solvent and the paper. Lastly, the blue spot traveled the furthest distance which was 11 cm and produced the highest RF value of 0.79.

[00:07:34]This suggest that the blue dye had a stronger affinity toward the mobile face and therefore move more easily along the paper. Overall the results showed that different food diet had a different migration behavior due to difference in their solubility and interaction with the station stationary and mobile phase.

[00:07:55]Paper chromatography successfully separated the food D and allow comparison using RF value.

[00:08:05]Now I will explain about the sample calculation that we use to determine the RF value. We use the formula RF equals to the distance traveled by the spot divided by the distance traveled by the solvent front. For the sample calculation, the blue spot was selected.

[00:08:25]From the result obtained, the blue dye traveled a distance of 11 cm while the solvent front travel 14 cm. So the calculation is RF equals to 11 divided by 14 which give an RF value of 0.79.

[00:08:43]This means the blue dye move approximately 79% of the total distance traveled by the solvent. A higher RF value indicates that the D has a stronger affinity toward the mobile phase which is the solvent and weaker interaction with the stationary face which is the chromatography paper.

[00:09:04]Therefore, the blue dice shows great mobility compared to the other dice in this experiment.

[00:09:14]My name is Fatina my student ID 202478433.

[00:09:20]Today I will discuss about discussion for our topic analysis of food dyes in M&M's using paper chromatography.

[00:09:29]So the paper chromatography experiment successfully separates the food dye from M&M candies. Each dye travel different due to difference in solubility in the solvent and attraction to the chromatography paper. So the solvent fronts moved a total of 14 cm. The blue dye traveled the further 11 cm followed by the yellow dye 8 cm with the red dye remain the origin 0 cm. The RF value calculate were blue uh 0.79, yellow 0.57 and red is 0.00. So the results show that the blue dye has the highest solubility in the mobile phase while the red dye has the strongest iteration to the stationary phase. The yellow dye show intermediate properties. H overall paper chromatography effectively separate the dye based on the chemical properties accuracy of the result.

[00:10:33]H maybe you guys still wondering what is the conclusion about this analysis. So today I would like to talk about that.

[00:10:40]So conclusion the paper chromatography experiment successfully separate the food dye from M&M candy and show that the different dye have different RF value due to the varying solubility and interaction with the stationary phase.

[00:10:53]The calculated of RF value were blue is 0.79 yellow is 0.57 and red 0.0 zero and indicating that the blue dye moved to the furthest while the red dye did not move at all. Overall paper chromatography is an effective method for separating and identifying component in a mixture based on the chemical properties.