Biotechnology encompasses techniques like genetic engineering, PCR, and recombinant DNA technology, which enable scientists to modify organisms by introducing, removing, or altering genes to achieve specific purposes such as disease treatment, crop improvement, and industrial applications. Key concepts include DNA replication mechanisms, vector systems (plasmids, bacterial artificial chromosomes), restriction enzymes, and the applications of these technologies in medicine (insulin production, gene therapy), agriculture (Bt cotton, Golden Rice), and environmental remediation.
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Biotechnology Unit test | Menti Quiz Live
Added:Welcome all to the live session. Welcome all.
Quiz started. Quiz started. Okay, now.
Quiz started.
Okay, now. So, quiz started.
First question of the day. In a key first question biotechnology unit test lab first question [snorts] Genetic engineering aims at destroying wild gene, replacing defective gene, curing human disease by introducing new gene, all the above.
All the above is the correct answer.
All the above is the correct answer.
Second question.
Hi. Hi. Hi. Welcome all. Welcome all to the live session.
Select the wrong statement.
Developing a DNA vaccine is an example of biotechnology.
First artificial recombinant DNA molecule was constructed in 1972.
Replication of DNA initiates at promoter site. Replication of plasmid is independent of the control of chromosomal DNA in bacteria.
Okay, replication of DNA initiates at promoter site in the top. Replication of DNA already region of the origin So I did that wrong statement.
Next question.
>> [snorts] >> Question of the day.
Good evening all. Welcome all.
Time of 30 seconds I increase 120 select the mismatched pair.
>> [snorts] >> Bio listings gold coated DNA microinjection antigen antibody reaction gene gun tungsten coated DNA PCR gene amplification. Mismatched pair the bottom pair I can do with you.
Okay microinjection it only Elisa antigen antibody reaction Elisa and one again. So 175 students wrong match I can do with you fourth question of the day.
Which of the following results in growth of desired organism in biotechnology?
Genetic engineering maintenance of sterile Indians and surrounding.
Immediate surrounding use of restriction enzyme all of the above.
Okay all of the above is the correct answer 176 students correct answer with you.
Fifth question of the day.
Which vector can clone only a small fragment of DNA?
Is artificial chromosome of plasmid or cosmid or bacterial artificial chromosome in the area?
Small fragment of DNA clone for the movie.
In the vector.
Plasmid. Plasmid is the vector. Okay now vector now the plasmid 173 students correct answer with you sixth question of the day.
Bio [snorts] ballistic technique is used in germplasm conservation process, tissue culture process, hybridization process, gene transfer process.
Gene transfer process is the correct answer.
Seventh question of the day.
Dash proteins involved in the replication of the plasmid.
Rop, helicase, polymerase, DNA dependent polymerase.
>> [snorts] >> Replication of plasmid protein, replication of plasmid.
Eighth question.
Key tools to be involved in recombinant DNA technology are A, ligase enzyme. B, polymerase enzyme.
C, ligase enzyme. D, vectors. A only, A and C only, A B C only, all of these.
Another key ecology unit 98:30.
Okay, [snorts] all of this is the correct answer. You have 204 students correct answer. Very good.
Another key ecology unit test 8:30 p.m.
Monday, human physiology unit test.
Okay.
Ninth The of the day.
All of the following are features required to facilitate cloning into a vector except nutrient medium cloning sites, selectable marker, origin of replication.
Cloning good and good and good and good and good and good and good and Okay, nutrient medium 10th question of the day.
Assertion: Agrobacterium tumefaciens is able to deliver a piece of DNA to transform normal plant cells into a tumor.
Reason: TI plasmid infects dicot plants.
Both assertion and reason true and reason is correct explanation of assertion and option one Reason is not correct explanation and option two. Assertion true reason false not third, assertion false reason true Okay. Both assertion reason both are true and reason explains assertion Okay.
8,248 575 BSE second mechanical Sanghavi third mechanical Okay, people look forward to come back.
Katie and I will name it up a market.
Okay.
Follow our wellness on the mutual and the vehicle in the back as your teacher ampicillin are in vector PBR322 indicates Okay.
ampicillin gene conferring resistance against ampicillin palindrome DNA sequence for EcoR1 ampicillin hybrid DNA Okay. Gene conferring resistance against ampicillin 12th question Oh, 12th question skipped by the children.
Okay, the 12th question.
In PBR322, [clears throat] BamH1 site is located in ampicillin resistant gene or tetracycline resistant gene or between ampicillin and tetracycline resistant gene or in both ampicillin and tetracycline resistant genes 194 students tetracycline resistant gene 13th question DNA is a one molecule dash molecule and it is dash past the plasma membrane hydrophilic or and cannot hydrophilic cannot uh hydrophobic it can pass the plasma membrane. Hydrophobic cannot say it is [clears throat] a hydrophilic molecule and cannot pass through the plasma membrane up to get the correct answer second option.
14th question Complete test one.
Complete test one.
Which of the following is not correctly matched with its use? Taq polymerase DNA polymerase used in polymerase chain reaction. Gene gun used for introducing recombinant DNA into plant cell.
Ethidium bromide stain okay. Fourth option is not correctly matched. Meaning one correct or not matched here.
To precipitate protein correct or not DNA.
To precipitate DNA. Okay. 169 students correct answer this question.
Question number 15.
Okay.
15th question.
Statement one Asexual reproduction is quite good with regard to biotechnology.
Statement two it is quite fast and rapid and it is also preserves the genetic complement.
>> Okay.
Both statement one >> [clears throat] >> 16th question Identify the correct statement.
DNA has a negatively charged phosphate backbone. DNA has a positively charged phosphate backbone.
When DNA is subjected to gel electrophoresis, it always travels towards cathode.
Um anode.
Okay.
17th Okay. Biolistic is suitable for transformation of plant cells.
18th question 18th question DNA cannot pass through a cell membrane as it is too big to cross the membrane. It is hydrophilic molecule. Membrane does not have specific protein to facilitate transport. None of the above.
Okay. It is hydrophilic molecule. Cell membrane on the hydrophobic membrane.
19th question Yes, it will fine.
The term competent refers to compete Okay, DNA 200 students correct answer but it's okay.
It is a hydrophilic molecule 19th question back up.
The term competent refers to increasing the efficiency with which DNA enters bacterium through pores in its cell wall.
187 students correct answer but it's okay. 20th question 20th question Your gene amplification can be done by polymerase chain reaction. As a cell number that reason in PCR multiple copies of gene of interest is synthesized.
Okay.
219 students both accession and reason are true. Reason is correct explanation of accession and correct answer but it's okay. Leader board back up.
Okay. Sanghavi first come again.
And the Donald Trump Cynthia Sri Perumal Top 10 and welcome. Welcome.
>> Okay.
Okay.
21st question.
Okay.
Recombinant DNA bearing ampicillin resistant gene in is passed in E. coli.
The latter are spread on agar plates containing ampicillin then transformed recipient cells grow and untransformed cells dying Okay, 22nd question. Okay.
The colonies of recombinant bacteria appears white in contrast to blue colonies of non-recombinant bacteria because of non-recombinant bacteria containing beta glucosidase.
Insertional inactivation of beta galactosidase in recombinant bacteria.
Insertional in Okay, second option. Insertional inactivation of beta galactosidase in recombinant recombinant bacteria.
Okay, Another recombinant bacteria white towel.
Non-recombinant blue hour look at the kind of second option in first inactivation of beta galactosidase in recombinant bacteria.
23rd question.
An ideal cloning vector should have a lower molecular weight, B origin of replication, C desired genes, D resistance to antibiotics. C and D but to my A B D A A and C A B and C A.
Ideal cloning vector came in a lot of them. A B C D left.
A B and D.
Desired gene cloning vector to come in a lot of them. Foreign DNA that desired gene to come okay. 98 students correct answer but it can get.
24th question.
In your polymerase chain reaction temperature required by denaturation 94 degree, annealing 40 degree, extension 72 degree ah. Meaning a lot of them but it can get correct option to come in a lot of them.
You look at the correct sequence in a lot of them but it can get denaturation, annealing, extension with the correct sequence.
Okay, denaturation and oh answer so it can get in there. And the temperature meaning a lot of them but it can get correct answer but it can get in there.
Biotechnology and its application in the statement one in polymerase chain reaction that polymerase is used.
Statement two that polymerase is a specific DNA polymerase which is thermostable.
26th question in the biotechnology application on a beacon.
Okay, both statements are correct.
26th question 50% of the quiz across Crystals of BT toxin produced by some bacteria do not kill bacteria themselves because bacteria are resistant to the toxin.
Toxin is immature. Toxin is inactive.
Bacteria encloses toxin in a special sack.
Okay, toxin is inactive.
In alkali gut cooler for another alkali pH level active up. Protoxin up.
27th question Golden rice A GM rice, B rich in vitamin A, C prevents night blindness, D prevent rickets. The correct article the inner golden rice or a character in that.
ABCD not a mirror my ABC matter what my BCD or my CND only one month.
>> [snorts] >> A B and C only 28th question All are biotechnological applications in order to increase food production except either biotechnology application career.
Bioremediation agrochemical based agriculture organic farming up genetically engineered crop based Bioremediation is the correct answer.
143 students correct answer.
29th question.
Insect resistance jam transgenic cotton has been produced by inserting a piece of DNA from an insect, a bacterium, a wild relative of cotton, a virus.
A bacterium is the correct answer. 186 students correct answer. Yeah.
Which of the following combination of risk are associated with genetically modified food?
Okay.
We're going to my answer with you. All of the above is the correct answer.
Okay. Toxicity allergic reaction to come. Antibiotic resistance in microorganisms.
Uh present in elementary canal. All of the above.
Leaderboard back line.
>> [snorts] >> Sanghavi Soul of Medical Shiva 31st question Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produces both sense and antisense RNA, a particular hormone, an antifeedant, a toxic protein.
Both sense and antisense RNA 32nd question of the day.
The genetically modified cotton in India has been developed for insect resistance, enhancing shelf life, enhancing mineral content, drought resistance.
Okay, insect resistance is the correct answer.
33rd question of the day.
By genetic modification of plants, mineral usage efficiency of plant can be dash and thus the exhaustion of fertility of soil is dash. Increased hastened hastened faster increased delayed uh decreased hastened decreased delayed delayed opposite hastened quick increased and soil uh fertility 34th An improved variety of transgenic golden rice is completely resistant to all insect pest and disease of paddy.
Gives high yield but no characteristic aroma.
Does not require chemical fertilizers and growth hormones. Give high yield and is rich in vitamin A.
211 students high yield and rich in rich in vitamin 35th question back now.
Statement one, totipotency is an artificially induced phenomena.
Statement two, the mammalian gametes is also known to possess totipotent traits.
Correct option is the winning candidate.
menti.com open browser layout I look forward to getting sign in sign up 711 85 884 join quiz Okay.
Totipotency artificially induced the final statement in natural uh you look totipotency natural capacity of any living plant cell Okay.
The mammalian gametes is also known as to promote possess totipotent mammalian I'm going to get animals animals cells to totipotency about 30 students answer note very lowest number Be very careful. Note it down. Okay.
36th question.
Which of the following pairs incorrectly matched?
Are the characteristics of insulin?
Pro-hormone contains C-peptide. Allergic reactions caused by foreign proteins in insulin. Mature insulin functional form.
Adenosine deaminase gene therapy.
Incorrect I believe in the mother.
Allergic reactions when the caused by foreign protein in insulin that is incorrect.
37th question but I am.
Okay, fourth option both are wrong and I let you get it in here. I don't think you have that silly mistake in something. The BT toxin is not toxic to human beings because Yeah, I bring it up there. Not option that is correct answer and I can do it again.
Okay. Conversion of pro BT toxin to BT toxin takes place only in highly alkaline condition that is under nematode and the pro water.
Ball worm water larva water gut. Okay.
I don't know my confession and I don't know I got to acidic I don't know.
38 degree.
The C-peptide is insulin that I call you a b c and the C-peptide. Not present in proinsulin. Present in mature insulin.
Removed during maturation of insulin.
Also present in artificial insulin.
Either correct or wrong.
Okay, removed during maturation of insulin.
39th question.
The early molecular diagnosis can be done by PCR, ELISA, Widal test, both one and two.
Both one and two. PCR polymerase chain reaction.
ELISA random error.
As a saying, we can produce transgenic bacteria to yield desired products.
Reason, human insulin can be produced by bacteria.
>> [snorts] >> Okay, both assertion and reason are true. Reason is correct explanation of assertion.
Varshini Rajendran first learn so love medical Shiva second learn again.
Sanghavi third looking at last 10 questions but they're all 41. Question number 41.
Extra time make a lump up.
for effective treatment of your disease.
Yearly diagnosis is required.
Sorry.
Yearly [snorts] diagnosis is required, but understanding of its pathophysiology is not required. Yearly diagnosis not required in the good.
188 students Third option yearly diagnosis and understanding of its pathophysiology are required.
>> [snorts] >> Okay, 42nd question.
Which of the following is not a true statement regarding BT cotton?
BT toxin is produced by a bacterium bacillus thuringiensis. It is an example of biopesticide. BT toxin gives a gene has both cloned in plant to provide resistance to fungal diseases.
BT cotton could decrease the amount of use of pesticide. Third option, BT toxin gene has been cloned in plants to provide resistance to fungal disease of being a the not true.
43rd question.
Transgenic crops are modified through genetic engineering to develop natural resistance to insect pest.
Which one is a transgenic plant?
Maize and sugarcane are tomato and wheat.
It is a year one day.
Transgenic plant tobacco and cotton is the correct answer.
44th question.
The genetic defect adenosine deaminase deficiency may be cured permanently by enzyme replacement therapy periodic infusion of genetically engineered lymphocytes having functional ADA cDNA or administering adenosine deaminase activators introducing bone marrow cells producing ADA into cells at early embryonic stage in the answer fourth option 190 for students correct answer in here.
Question number 45 but Statement one human insulin can be produced by protozoa. Statement two insulin from different animals causes allergic reaction in humans.
In the option correct and kind of it can do.
Okay, statement one is incorrect.
Statement two is correct and kind of it correct answer 46 question but now Golden rice developed through transgene approach is enriched with high lysine content high methionine content or high glutamine content or high vitamin A content or High vitamin A content is the correct answer.
47th question Transgenic plants are developed by >> plants are developed by introducing foreign genes, introducing gene mutations, deleting certain chromosome parts, stopping spindle formation.
Okay, introducing foreign genes is the correct answer. You are not able to give the correct answer for anything.
48th question Transgenic models exist for all the following diseases >> [snorts] >> Hypertension is not a human hypertension color at all.
Animals hypertension is not there.
And genetics related hypertension stress related food habit related stress related. Okay, biopatterns are right to use invention, right to use products, right to use biological entities, right to use process. You and see what do you see only A, C, D or all of these?
Okay, all of this is the correct answer.
154 students correct answer again.
50th question, last question of the day.
Now I can unit test 5 ecology 8:30 p.m. Assassin business forms protein crystals during particular phase of growth. Reason cryoproteins are soluble in alkaline environment of mid gut of insect. [snorts] >> [snorts]
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